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Restoring mitochondrial function and normalizing ROS‐JNK/MAPK pathway exert key roles in glutamine ameliorating bisphenol A‐induced intestinal injury
Author(s) -
Jiao Ning,
Xu Doudou,
Qiu Kai,
Wang Liqi,
Wang Lu,
Piao Xiangshu,
Yin Jingdong
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201902503r
Subject(s) - glutamine , mapk/erk pathway , apoptosis , mitochondrion , mitochondrial ros , programmed cell death , biology , microbiology and biotechnology , chemistry , endocrinology , kinase , biochemistry , amino acid
Bisphenol A (BPA) is toxic to the reproductive and nervous system, even carcinogenetic in humans and animals. However, few studies focused on effects of BPA on the intestinal tract. Here, we detected BPA‐induced injuries on intestinal mucosa and explored a reliable approach to counteract BPA effects. C57BL/6J mice were gavage BPA or BPA accompanied with ingestion of 4% (w/w) of glutamine for 4‐wks. In vitro, IEC‐6 cells were treated with 0.4 mmol/L BPA for 6 hours mimicking acute injury and 0.2 mmol/L BPA for 12 hours followed with or without the inclusion of 4 mmol/L glutamine for 12 hours to determine cell renewal, mitochondrial function and ROS‐JNK/MAPK pathway upon moderate BPA exposure. As results, BPA exposure caused severe intestinal injury, and disturbed intestinal epithelial cell proliferation and apoptosis, accompanied with mitochondrial malfunction and activated JNK/MAPK pathway as well. Notably, glutathione metabolism was implicated in BPA‐induce injury. Glutamine could well rescue cell renewal and mitochondrial function from BPA exposure‐induced injuries. In conclusion, we demonstrated impaired effect of BPA exposure on intestinal functions, which could be well counteracted by glutamine partly via restoring mitochondrial function and normalizing ROS‐JNK/MAPK pathway. Thereby, we provided a novel application of glutamine to rescue intestinal injury.