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LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization
Author(s) -
Li Xueqin,
Zhang Yingying,
Pei Weiya,
Zhang Mengying,
Yang Hui,
Zhong Min,
Kong Xiang,
Xu Yang,
Zhu Xiaolong,
Chen Tianbing,
Ye Jingjing,
Lv Kun
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201902379r
Subject(s) - macrophage polarization , dna methylation , gene knockdown , dna methyltransferase , methylation , methylated dna immunoprecipitation , biology , dna , microbiology and biotechnology , macrophage , methyltransferase , gene expression , gene , genetics , in vitro
Abstract Long non‐coding RNAs (lncRNAs) play key roles in various biological processes. However, the roles of lncRNAs in macrophage polarization remain largely unexplored. In this study, thousands of lncRNAs were identified that are differentially expressed in distinct polarized bone marrow‐derived macrophages. Among them, Dnmt3aos (DNA methyltransferase 3A, opposite strand), as a known lncRNA, locates on the antisense strand of Dnmt3a . Functional experiments further confirmed that Dnmt3aos were highly expressed in M(IL‐4) macrophages and participated in the regulation of Dnmt3a expression, and played a key role in macrophage polarization. The DNA methylation profiles between the Dnmt3aos knockdown group and the control group in M(IL‐4) macrophages were determined by MeDIP‐seq technique for the first time, and the Dnmt3aos‐Dnmt3a axis‐mediated DNA methylation modification‐regulated macrophage polarization‐ related gene IFN‐γ was identified. Our study will help to enrich our knowledge of the mechanism of macrophage polarization.