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Individual and combined effects of the infrared, visible, and ultraviolet light components of solar radiation on damage biomarkers in human skin cells
Author(s) -
Hudson Laura,
Rashdan Eyman,
Bonn Catherine A.,
Chavan Bhaven,
Rawlings David,
BirchMachin Mark A.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201902351rr
Subject(s) - photoaging , ultraviolet , dna damage , photoprotection , human skin , dermal fibroblast , reactive oxygen species , visible spectrum , biophysics , infrared , chemistry , sunlight , cell culture , fibroblast , materials science , optoelectronics , dna , biology , dermatology , medicine , optics , genetics , biochemistry , physics , photosynthesis
The ability of solar ultraviolet (UV) to induce skin cancer and photoaging is well recognized. The effect of the infrared (IR) and visible light (Vis) components of solar radiation on skin and their interaction with UV is less well known. This study compared the effects of physiologically relevant doses of complete (UV + Vis + IR) solar‐simulated light and its individual components on matched primary dermal fibroblasts and epidermal keratinocytes from human donors on three biomarkers of cellular damage (reactive oxygen species (ROS) generation, mitochondrial DNA (mtDNA), and nuclear DNA (nDNA) damage). There was a greater induction of ROS, mtDNA, and nDNA damage with the inclusion of the visible and IR components of solar‐simulated light in primary fibroblast cells compared to primary keratinocytes ( P < .001). Experiments using exposure to specific components of solar light alone or in combination showed that the UV, Vis, and IR components of solar light synergistically increased ROS generation in primary fibroblasts but not primary keratinocytes ( P < .001). Skin cell lines were used to confirm these findings. These observations have important implications for different skin cell type responses to the individual and interacting components of solar light and therefore photodamage mechanisms and photoprotection interventions.