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Adipose‐derived stem cells integrate into trabecular meshwork with glaucoma treatment potential
Author(s) -
Zhou Yi,
Xia Xiaobo,
Yang Enzhi,
Wang Yiwen,
Marra Kacey G.,
Ethier C. Ross,
Schuman Joel S.,
Du Yiqin
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201902326r
Subject(s) - trabecular meshwork , stem cell , glaucoma , adipose tissue , intraocular pressure , homing (biology) , mesenchymal stem cell , transplantation , medicine , microbiology and biotechnology , pathology , biology , ophthalmology , ecology
The trabecular meshwork (TM) is an ocular tissue that maintains intraocular pressure (IOP) within a physiologic range. Glaucoma patients have reduced TM cellularity and, frequently, elevated IOP. To establish a stem cell‐based approach to restoring TM function and normalizing IOP, human adipose‐derived stem cells (ADSCs) were induced to differentiate to TM cells in vitro. These ADSC‐TM cells displayed a TM cell‐like genotypic profile, became phagocytic, and responded to dexamethasone stimulation, characteristic of TM cells. After transplantation into naive mouse eyes, ADSCs and ADSC‐TM cells integrated into the TM tissue, expressed TM cell markers, and maintained normal IOP, outflow facility, and extracellular matrix. Cell migration and affinity results indicated that the chemokine pair CXCR4/SDF1 may play an important role in ADSC‐TM cell homing. Our study demonstrates the possibility of applying autologous or allogeneic ADSCs and ADSC‐TM cells as a potential treatment to restore TM structure and function in glaucoma.