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A centrosome‐localized calcium signal is essential for mammalian cell mitosis
Author(s) -
Helassa Nordine,
Nugues Charlotte,
Rajamanoharan Dayani,
Burgoyne Robert D.,
Haynes Lee P.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201901662r
Subject(s) - mitosis , centrosome , microbiology and biotechnology , endoplasmic reticulum , biology , microtubule , spindle apparatus , calcium signaling , calcium , cell , chemistry , cell cycle , cell division , signal transduction , biochemistry , organic chemistry
Mitosis defects can lead to premature ageing and cancer. Understanding mitosis regulation therefore has important implications for human disease. Early data suggested that calcium (Ca 2+ ) signals could influence mitosis, but these have hitherto not been observed in mammalian cells. Here, we reveal a prolonged yet spatially restricted Ca 2+ signal at the centrosomes of actively dividing cells. Local buffering of the centrosomal Ca 2+ signals, by flash photolysis of the caged Ca 2+ chelator diazo‐2‐acetoxymethyl ester, arrests mitosis. We also provide evidence that this Ca 2+ signal emanates from the endoplasmic reticulum. In summary, we characterize a unique centrosomal Ca 2+ signal as a functionally essential input into mitosis.—Helassa, N., Nugues, C., Rajamanoharan, D., Burgoyne, R. D., Haynes, L. P. A centrosome‐localized calcium signal is essential for mammalian cell mitosis. FASEB J. 33, 14602‐14610 (2019). www.fasebj.org