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Using 17β‐estradiol heparin‐poloxamer thermosensitive hydrogel to enhance the endometrial regeneration and functional recovery of intrauterine adhesions in a rat model
Author(s) -
Zhang SiSi,
Xu XinXin,
Xiang WeiWei,
Zhang HuiHeng,
Lin HuiLong,
Shen LaiEn,
Lin Qi,
Lin Feng,
Zhou ZhiYang
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201901603rr
Subject(s) - endometrium , medicine , regeneration (biology) , uterine cavity , self healing hydrogels , chemistry , andrology , uterus , microbiology and biotechnology , biology , organic chemistry
Mechanical damage or infection to the endometrium can lead to the formation of adhesions in the uterine cavity, which may result in reduced reproductive outcome and/or pregnancy complications. The prognosis of this disease is poor due to few effective treatments and the complex environment of endometrium. Heparin‐Poloxamer Hydrogel (HP hydrogel) is a nontoxic and biodegradable biomaterial, which has been commonly used as a sustained‐release delivery system. In this study, we applied a mini‐endometrial curette to scrape the endometrium of rats to mimic the process of curettage in patients. After the establishment of IUA model in rats, we injected the thermo‐sensitive hydrogel(E 2 ‐HP hydrogel) into the injured uterine cavity and evaluated the therapeutic effect of E 2 ‐HP hydrogel on the recovery of IUA. Our results showed that E 2 ‐HP hydrogel can significantly facilitate the regeneration of injured endometrium along with inhibiting the cell apoptosis in IUA model. Furthermore, we revealed that E 2 ‐HP hydrogel on the recovery of IUA was closely associated with the upregulation of kisspeptin through activating the ERK1/2 and MAPKs p38 pathways. In conclusion, E 2 ‐HP hydrogel can effectively transfer E 2 into the injured endometrium and it can be considered as a promising therapeutic method for the women with intrauterine adhesions.