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Evaluation of oxidative stress mechanisms and the effects of phytotherapic extracts on Parkinson's disease Drosophila PINK1 B9 model
Author(s) -
Baroli Biancamaria,
Loi Eleonora,
Solari Paolo,
Kasture Ameya,
Moi Loredana,
Muroni Patrizia,
Kasture Sanjay,
Setzu Maria Dolores,
Liscia Anna,
Zavattari Patrizia
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201901010
Subject(s) - oxidative stress , pink1 , superoxide dismutase , parkinson's disease , withania somnifera , biology , glutathione , glutathione reductase , drosophila melanogaster , pharmacology , biochemistry , parkin , medicine , glutathione peroxidase , disease , enzyme , pathology , gene , alternative medicine
Oxidative stress is commonly observed in both idiopathic and genetic cases of Parkinson's disease (PD). It plays an important role in the degeneration of dopaminergic neurons, and it has been associated with altered telomere length (TL). There is currently no cure for PD, and extracts of antioxidative plant, such as Mucunα pruriens and Withania somnifera , are commonly used in Ayurveda to treat patients with PD. In this study, we evaluated 2 enzymatic markers of oxidative stress, glutathione (GSH) system and superoxide dismutase (SOD), and TL in a Drosophila melanogaster model for PD [phosphatase and tensin homolog‐induced putative kinase 1 ( PINK1 ) B9 ]. This evaluation was also performed after treatment with the phytoextracts. PINK1 B9 mutants showed a decrease in GSH amount and SOD activity and unexpected longer telomeres compared with wild‐type flies. M. pruriens treatment seemed to have a beneficial effect on the oxidative stress conditions. On the other hand, W. somnifera treatment did not show any improvements in the studied oxidative stress mechanisms and even seemed to favor the selection of flies with longer telomeres. In summary, our study suggests the importance of testing antioxidant phytoextracts in a PINK1 B9 model to identify beneficial effects for PD.—Baroli, B., Loi, E., Solari, P., Kasture, A., Moi, L., Muroni, P., Kasture, S., Setzu, M. D., Liscia, A., Zavattari, P. Evaluation of oxidative stress mechanisms and the effects of phytotherapic extracts on Parkinson's disease Drosophila PINK1 B9 model. FASEB J. 33, 11028–11034 (2019). www.fasebj.org