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Crosstalk between soluble PDGF‐BB and PDGFRβ promotes astrocytic activation and synaptic recovery in the hippocampus after subarachnoid hemorrhage
Author(s) -
Zhou Xiaoming,
Wu Qi,
Lu Yue,
Zhang Xiangsheng,
Lv Shengyin,
Shao Jiang,
Zhou Yuan,
Chen Jigang,
Hou Lijun,
Huang Chengguang,
Zhang Xin
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201900195r
Subject(s) - platelet derived growth factor receptor , subarachnoid hemorrhage , neuroprotection , growth factor , astrocyte , platelet derived growth factor , neuroscience , medicine , chemistry , microbiology and biotechnology , endocrinology , biology , receptor , central nervous system
Platelet‐derived growth factor receptor β (PDGFRβ) dynamically changes after brain injury, possibly mediating the neuroprotective role of soluble homodimers of the platelet‐derived growth factor β subunit (PDGF‐BB) that is secreted by microcirculation cells. The aim of this study was to determine whether binding of PDGF‐BB to astrocytic PDGFRβ enhanced crosstalk among the various components of the neurovascular unit, leading to synaptic recovery after subarachnoid hemorrhage (SAH). The soluble PDGF‐BB from the cerebrospinal fluid (CSF) of patients with SAH was measured. The relationship between PDGF‐BB treatment and astrocytic PDGFRβ signaling was further explored in vivo and in vitro in experimental SAH models. Compared with the levels in the control samples, the PDGF‐BB protein levels in the CSF of patients with SAH were significantly increased. After the generation of experimental SAH, astrocyte activation markers were markedly induced by the binding of PDGF‐BB to astrocytic PDGFRβ, accompanied by improved levels of synaptic recovery and cognitive function. Soluble PDGF‐BB and astrocytic PDGFRβ signaling are essential for the neuroprotective effect in the hippocampus and the coculture system in vitro after SAH that otherwise leads to cognitive dysfunction and neuronal damage.—Zhou, X., Wu, Q., Lu, Y., Zhang, X., Lv, S., Shao, J., Zhou, Y., Chen, J., Hou, L., Huang, C., Zhang, X. Crosstalk between soluble PDGF‐BB and PDGFRβ promotes astrocytic activation and synaptic recovery in the hippocampus after subarachnoid hemorrhage. FASEB J. 33, 9588–9601 (2019). www.fasebj.org