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Coalescing replication compartments provide the opportunity for recombination between coinfecting herpesviruses
Author(s) -
Tomer Enosh,
Cohen Efrat M.,
Drayman Nir,
Afriat Amichay,
Weitzman Matthew D.,
Zaritsky Assaf,
Kobiler Oren
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201900032r
Subject(s) - recombination , genome , biology , herpes simplex virus , homologous recombination , viral replication , herpesviridae , dna , dna replication , virology , genetics , virus , gene , viral disease
Homologous recombination (HR) is considered a major driving force of evolution because it generates and expands genetic diversity. Evidence of HR between coinfecting herpesvirus DNA genomes can be found frequently both in vitro and in clinical isolates. Each herpes simplex virus type 1 (HSV‐1) replication compartment (RC) derives from a single incoming genome and maintains a specific territory within the nucleus. This raises intriguing questions about where and when coinfecting viral genomes interact. To study the spatiotemporal requirements for intergenomic recombination, we developed an assay with dual‐color FISH that enables detection of HR between different pairs of coinfecting HSV‐1 genomes. Our results revealed that HR increases intermingling of RCs derived from different genomes. Furthermore, inhibition of RC movement reduces the rate of HR events among coinfecting viruses. Finally, we observed correlation between nuclear size and the number of RCs per nucleus. Our findings suggest that both viral replication and recombination are subject to nuclear spatial constraints. Other DNA viruses and cellular DNA are likely to encounter similar restrictions.—Tomer, E., Cohen, E. M., Drayman, N., Afriat, A., Weitzman, M. D., Zaritsky, A., Kobiler, O. Coalescing replication compartments provide the opportunity for recombination between coinfecting herpesviruses. FASEB J. 33, 9388–9403 (2019). www.fasebj.org