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Vitamin D improves endothelial barrier integrity and counteracts inflammatory effects on endothelial progenitor cells
Author(s) -
SchröderHeurich Bianca,
Hardenberg Sandra,
Brodowski Lars,
Kipke Berina,
Meyer Nadia,
Borns Katja,
Kaisenberg Constantin S.,
Brinkmann Hella,
Claus Peter,
VersenHöynck Frauke
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201802750rr
Subject(s) - progenitor cell , microbiology and biotechnology , endothelial stem cell , ve cadherin , endothelial progenitor cell , angiogenesis , chemistry , endothelium , cadherin , biology , cell , stem cell , cancer research , biochemistry , endocrinology , in vitro
Endothelial colony‐forming cells (ECFCs), a proliferative subpopulation of endothelial progenitor cells, are involved in angiogenesis and endothelial repair. In this study, we investigated endothelial barrier characteristics of ECFCs, whether vitamin D supports cell‐cell adhesion and barrier integrity, and how it affects ECFC mobilization and actin dynamics. Although ECFC barrier was disrupted under inflammatory conditions, this effect was rescued by vitamin D treatment, leading to higher stability of an ECFC monolayer. Furthermore, vitamin D enhanced ECFC mobilization toward directional migration. In addition, immunocytochemistry, quantitative real‐time PCR, and immunoblotting analysis showed that vitamin D increased endothelial interconnections through vascular endothelial Cadherin (VE‐cadherin) junctions and by impacting cell dynamics through cofilin and VE‐cadherin phosphorylation. Our results suggest that vitamin D treatment efficiently counteracts inflammation in an ECFC monolayer, resulting in higher ECFC barrier integrity. This study provides evidence of a new beneficial effect of vitamin D for ECFC homeostasis. —Schröder‐Heurich, B., von Hardenberg, S., Brodowski, L., Kipke, B., Meyer, N., Borns, K., von Kaisenberg, C. S., Brinkmann, H., Claus, P., von Versen‐Höynck, F. Vitamin D improves endothelial barrier integrity and counteracts inflammatory effects on endothelial progenitor cells. FASEB J. 33, 9142–9153 (2019). www.fasebj.org