Premium
Maternal obesity results in decreased syncytiotrophoblast synthesis of palmitoleic acid, a fatty acid with anti‐inflammatory and insulin‐sensitizing properties
Author(s) -
FerchaudRoucher Véronique,
Barner Kelsey,
Jansson Thomas,
Powell Theresa L.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201802444r
Subject(s) - palmitoleic acid , endocrinology , medicine , syncytiotrophoblast , insulin resistance , linoleic acid , dyslipidemia , fatty acid , chemistry , oleic acid , docosahexaenoic acid , lipid metabolism , insulin , obesity , fetus , biology , placenta , biochemistry , polyunsaturated fatty acid , pregnancy , genetics
The fetus is dependent on delivery of fatty acids (FAs) by the syncytiotrophoblast, the transporting epithelium of the human placenta. Obese pregnant women have dyslipidemia; however, whether obesity impacts placental lipid transport and metabolism remains to be fully established. Palmitoleic acid (POA), an FA with antiinflammatory and insulin‐sensitizing properties, is synthesized from palmitic acid (PA) catalyzed by stearoyl‐coenzyme A desaturase (SCD) activity. We hypothesized that the uptake and incorporation of FAs and POA synthesis are reduced in primary human trophoblasts (PHTs) isolated from pregnancies complicated by maternal obesity. Villous cytotrophoblasts were isolated from 7 placentas of obese [body mass index (BMI) = 37.5 ± 1.9] and 12 normal (BMI = 23.6 ± 0.6) mothers. FA uptake and incorporation were assessed using uniformly labeled (U[ 13 C])–FA mixtures of PA, oleic acid (OA), linoleic acid, and docosahexaenoic acid. Cellular [ 13 C] FAs were quantified both in total cellular lipids and in lipid classes by GC‐MS. Uptake and incorporation of [ 13 C] FAs in total cellular lipids were not different in PHTs isolated from obese mothers compared with normal mothers. Only the concentration of OA was increased in the triglyceride fraction ( P < 0.05) if the mother was obese. We found an isotopic enrichment of POA after U[ 13 C]‐PA treatment, demonstrating SCD activity in PHT cells. Labeled POA content and the POA:PA ratio were significantly lower in PHTs isolated from placentas of obese mothers compared with normal, healthy controls. Decreased syncytiotrophoblast POA synthesis may contribute to insulin resistance and low‐grade inflammation in the mother, placenta, or fetus (or a combination of the 3) in pregnancies complicated by obesity.—Ferchaud‐Roucher, V., Barner, K., Jansson, T., Powell, T. L. Maternal obesity results in decreased syncytiotrophoblast synthesis of palmitoleic acid, a fatty acid with anti‐inflammatory and insulin‐sensitizing properties. FASEB J. 33, 6643–6654 (2019). www.fasebj.org