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Spire localization via zinc finger—containing domain is crucial for the asymmetric division of mouse oocyte
Author(s) -
Jo Yu-Jin,
Lee In-Won,
Jung Seung-Min,
Kwon Jeongwoo,
Kim Nam-Hyung,
Namgoong Suk
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201801905r
Subject(s) - oocyte , cytokinesis , microbiology and biotechnology , biology , oocyte activation , actin , zinc finger , cell division , cytoplasm , cytoskeleton , ring finger domain , genetics , embryo , cell , transcription factor , gene
Zinc plays an essential role in mammalian oocyte maturation, fertilization, and early embryogenesis, and depletion of zinc impairs cell cycle control, asymmetric division, and cytokinesis in oocyte. We report that zinc, via the actin nucleator Spire, acts as an essential regulator of the actin cytoskeleton remodeling during mouse oocyte maturation and fertilization. Depletion of zinc in the mouse oocyte impaired cortical and cytoplasmic actin formation. Spire is colocalized with zinc‐containing vesicles via its zinc finger‐containing Fab1, YOTB, Vac 1, EEA1 (FYVE) domain. Improper localization of Spire by zinc depletion or mutations in the FYVE domain impair cytoplasmic actin mesh formations and asymmetric division and cytokinesis of oocyte. All 3 major domains of the Spire are required for its proper localization and activity. After fertilization or parthenogenetic activation, Spire localization was dramatically altered following zinc release from the oocyte. Collectively, our data reveal novel roles for zinc in the regulation of the actin nucleator Spire by controlling its localization in mammalian oocyte.—Jo, Y.‐J., Lee, I.‐W., Jung, S.‐M., Kwon, J., Kim, N.‐H., Namgoong, S. Spire localization via zinc finger—containing domain is crucial for the asymmetric division of mouse oocyte. FASEB J. 33, 4432–4447 (2019). www.fasebj.org