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Stress hormone signaling through β‐adrenergic receptors regulates macrophage mechanotype and function
Author(s) -
Kim Tae-Hyung,
Ly Chau,
Christodoulides Alexei,
Nowell Cameron J.,
Gunning Peter W.,
Sloan Erica K.,
Rowat And Amy C.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201801429rr
Subject(s) - microbiology and biotechnology , actin cytoskeleton , cytoskeleton , receptor , phagocytosis , actin , macrophage , immune system , signal transduction , hormone , stress fiber , macrophage migration inhibitory factor , cell signaling , biology , chemistry , immunology , cell , endocrinology , biochemistry , cytokine , focal adhesion , in vitro
Critical functions of immune cells require them to rapidly change their shape and generate forces in response to cues from their surrounding environment. However, little is known about how soluble factors that may be present in the microenvironment modulate key aspects of cellular mechanobiology—such as immune cell deformability and force generation—to impact functions such as phagocytosis and migration. Here we show that signaling by soluble stress hormones through β‐adrenoceptors (β‐AR) reduces the deformability of macrophages; this is dependent on changes in the organization of the actin cytoskeleton and is associated with functional changes in phagocytosis and migration. Pharmacologic interventions reveal that the impact of β‐AR signaling on macrophage deformability is dependent on actin‐related proteins 2/3, indicating that stress hormone signaling through β‐AR shifts actin organization to favor branched structures rather than linear unbranched actin filaments. These findings show that through remodeling of the actin cytoskeleton, β‐AR‐mediated stress hormone signaling modulates macrophage mechanotype to impact functions that play a critical role in immune response.—Kim, T.‐H., Ly, C., Christodoulides, A., Nowell, C. J., Gunning, P. W., Sloan, E. K., Rowat, A. C. Stress hormone signaling through β‐adrenergic receptors regulates macrophage mechanotype and function. FASEB J. 33, 3997–4006 (2019). www.fasebj.org