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Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows
Author(s) -
Zhao Fang,
Wu Wangjun,
Wei Quanwei,
Shen Ming,
Li Bojiang,
Jiang Yi,
Liu Kaiqing,
Liu Honglin
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201801081rrr
Subject(s) - dna methylation , adrenocorticotropic hormone , transcriptome , biology , gene , methylation , differentially methylated regions , epigenetics , corpus luteum , bisulfite sequencing , genome , genomic dna , genetics , gene expression , microbiology and biotechnology , endocrinology , medicine , hormone
Stress is known to cause corpus luteum (CL) dysfunction, and stress hormones play a critical role in this process. However, the mechanism remains unclear. In this study, weaned sows were injected with synthetic adrenocorticotropic hormone (ACTH) for 7 d; whole‐genome bisulfite sequencing (WGBS) and RNA sequencing was used respectively to investigate the systematic association between ACTH administration and DNA methylation in CL and its relationship to gene expression. Results showed that ACTH treatment significantly increased the concentrations of Cortisol ( P < 0.05). The genome‐wide DNA methylation maps of CL were provided, and the global analysis showed the difference between the 2 groups exists in the chromosomes and feature regions of the genome. A total of 88,559 DMRs were identified and the most DMR‐related genes were gathered in terms of metabolic biologic processes, and some DMR‐related genes were involved in cellular differentiation. Nine differentially expressed genes were screened out of coexpressed genes and 4 DMR‐associated genes that were also differentially expressed ( P < 0.05). In summary, our study firstly provides insight into the regulation of ACTH administration on genomic DNA methylation and gene expression in CL. We revealed a remarkable alteration of DNA methylation in CL caused by ACTH treatment, and identified 4 DMR‐related genes that may be involved in the CL function under stress conditions.—Zhao, F., Wu, W., Wei, Q., Shen, M., Li, B., Jiang, Y., Liu, K., Liu, H. Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows. FASEB J. 33, 3264–3278 (2019). www.fasebj.org