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HuR stabilizes a polyadenylated form of replication‐dependent histone mRNAs under stress conditions
Author(s) -
Ryu Incheol,
Park Yeonkyoung,
Seo Jwa-Won,
Park Ok Hyun,
Ha Hongseok,
Nam Jin-Wu,
Kim Yoon Ki
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201800431r
Subject(s) - polyadenylation , messenger rna , microbiology and biotechnology , translation (biology) , untranslated region , biology , histone , polysome , biogenesis , genetics , rna , gene , ribosome
All metazoan mRNAs have a poly(A) tail at the 3′ end with the exception of replication‐dependent histone (RDH) mRNAs, which end in a highly conserved stem‐loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A) + RDH] mRNAs remain unknown. In this study, our genome‐wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A) + RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A) + RDH mRNAs occurs in a translation‐independent manner and is regulated via human antigen R (HuR) binding to the extended 3′ UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A) + RDH mRNAs.—Ryu, I., Park, Y., Seo, J.‐W., Park, O. H., Ha, H., Nam, J.‐W., Kim, Y. K. HuR stabilizes a polyadenylated form of replication‐dependent histone mRNAs under stress conditions. FASEB J. 33, 2680–2693 (2019). www.fasebj.org