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Stabilized collagen matrix dressing improves wound macrophage function and epithelialization
Author(s) -
El Masry Mohamed S.,
Chaffee Scott,
Das Ghatak Piya,
Mathew-Steiner Shomita S.,
Das Amitava,
Higuita-Castro Natalia,
Roy Sashwati,
Anani Raafat A.,
Sen Chandan K.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201800352r
Subject(s) - medicine , center (category theory) , management , library science , chemistry , economics , computer science , crystallography
Decellularized matrices of biologic tissue have performed well as wound care dressings. Extracellular matrix‐based dressings are subject to rapid degradation by excessive protease activity at the wound environment. Stabilized, acellular, equine pericardial collagen matrix (sPCM) wound care dressing is flexible cross‐linked proteolytic enzyme degradation resistant. sPCM was structurally characterized utilizing scanning electron and atomic force microscopy. In murine excisional wounds, sPCM was effective in mounting an acute inflammatory response. Postwound inflammation resolved rapidly, as indicated by elevated levels of IL‐10, arginase‐1, and VEGF, and lowering of IL‐lβ and TNF‐α. sPCM induced antimicrobial proteins S100A9 and β‐defensin‐1 in keratinocytes. Adherence of Pseudomonas aeruginosa and Staphylococcus aureus on sPCM pre‐exposed to host immune cells in vivo was inhibited. Excisional wounds dressed with sPCM showed complete closure at d 14, while control wounds remained open. sPCM accelerated wound re‐epithelialization. sPCM not only accelerated wound closure but also improved the quality of healing by increased collagen deposition and maturation. Thus, sPCM is capable of presenting scaffold functionality during the course of wound healing. In addition to inducing endogenous antimicrobial defense systems, the dressing itself has properties that minimize biofilm formation. It mounts robust inflammation, a process that rapidly resolves, making way for wound healing to advance.—El Masry, M. S., Chaffee, S., Das Ghatak, P., Mathew‐Steiner, S. S., Das, A., Higuita‐Castro, N., Roy, S., Anani, R. A., Sen, C. K. Stabilized collagen matrix dressing improves wound macrophage function and epithelialization. FASEB J. 33, 2144–2155 (2019). www.fasebj.org