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Ecdysone‐responsive microRNA‐252‐5p controls the cell cycle by targeting Abi in Drosophila
Author(s) -
Lim DoHwan,
Lee Seungjae,
Yun Han Jee,
Choi MinSeok,
Hong JaeSang,
Seong Youngmo,
Kwon YoungSoo,
Sik Lee Young
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201701185rr
Subject(s) - ecdysone , biology , microrna , ecdysone receptor , microbiology and biotechnology , effector , cell cycle , gene , genetics , transcription factor , nuclear receptor
The steroid hormone ecdysone has a central role in the developmental transitions of insects through its control of responsive protein‐coding and microRNA (miRNA) gene expression. However, the complete regulatory network controlling the expression of these genes remains to be elucidated. In this study, we performed cross‐linking immunoprecipitation coupled with deep sequencing of endogenous Argonaute 1 (Agol) protein, the core effector of the miRNA pathway, in Drosophila S2 cells. We found that regulatory interactions between miRNAs and their cognate targets were substantially altered byAgo1inresponsetoecdysonesignaling. Additionally, during the larva‐to‐adult metamorphosis, miR‐252‐5p was up‐regulated via the canonical ecdysone‐signaling pathway. Moreover, we provide evidence that miR‐252‐5p targets Abelson interacting protein (Abi) to decrease the protein levels of cyclins A and B, controlling the cell cycle. Overall, our data suggest a potential role for the ecdysone/miR‐252‐5p/Abi regulatory axis partly in cell‐cycle control during metamorphosis in Drosophila .—Lim D.‐H., Lee, S., Han, J.Y., Choi, M.‐S., Hong J.‐S., Seong, Y., Kwon, Y.‐S., Lee Y.S. Ecdysone‐responsive microR‐252‐5p controls the cell cycle by targeting Abi in Drosophila . FASEB J . 32, 4519–4533 (2018). www.fasebj.org