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IKK2/NF‐κB signaling protects neurons after traumatic brain injury
Author(s) -
Mettang Melanie,
Reichel Stephanie Nadine,
Lattke Michael,
Palmer Annette,
Abaei Alireza,
Rasche Volker,
HuberLang Markus,
Baumann Bernd,
Wirth Thomas
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201700826r
Subject(s) - nf κb , iκb kinase , proinflammatory cytokine , signal transduction , neuroscience , inflammation , traumatic brain injury , regulator , forebrain , programmed cell death , kinase , apoptosis , medicine , microbiology and biotechnology , biology , immunology , central nervous system , gene , psychiatry , genetics
Traumatic brain injury (TBI) is the leading cause of death in young adults. After the initial injury, a poorly understood secondary phase, including a strong inflammatory response determines the final outcome of TBI. The inhibitor of NF‐κB kinase (IKK)/NF‐κB signaling system is the key regulator of inflammation and also critically involved in regulation of neuronal survival and synaptic plasticity. We addressed the neuron‐specific function of IKK2/NF‐κB signaling pathway in TBI using an experimental model of closed‐head injury (CHI) in combination with mouse models allowing conditional regulation of IKK/NF‐κB signaling in excitatory forebrain neurons. We found that repression of IKK2/NF‐κB signaling in neurons increases the acute posttraumatic mortality rate, worsens the neurological outcome, and promotes neuronal cell death by apoptosis, thus resulting in enhanced proinflammatory gene expression. As a potential mechanism, we identified elevated levels of the proapoptotic mediators Bax and Bad and enhanced expression of stress response genes. This phenotype is also observed when neuronal IKK/NF‐κB activity is inhibited just before CHI. In contrast, neuron‐specific activation of IKK/NF‐κB signaling does not alter the TBI outcome. Thus, this study demonstrates that physiological neuronal IKK/NF‐κBsignalingis necessary and sufficient to protect neurons from trauma consequences.— Mettang, M., Reichel, S.N., Lattke, M., Palmer, A., Abaei, A., Rasche, V., Huber‐Lang, M., Baumann, B., Wirth, T. IKK2/NF‐κB signaling protects neurons after traumatic brain injury. FASEB J. 32, 1916–1932 (2018). www.fasebj.org