Premium
Organic anion‐transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imaging
Author(s) -
Wu MenqRong,
Liu HonMan,
Lu ChenWen,
Shen WayHone,
Lin IJou,
Liao LiWen,
Huang YiYou,
Shieh MingJium,
Hsiao JongKai
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201700767r
Subject(s) - reporter gene , in vivo , magnetic resonance imaging , molecular imaging , chemistry , green fluorescent protein , fluorescence , organic anion transporter 1 , fluorescence lifetime imaging microscopy , indocyanine green , preclinical imaging , biophysics , microbiology and biotechnology , transporter , gene , biochemistry , biology , pathology , gene expression , medicine , physics , radiology , quantum mechanics
Reporter proteins have broad applications in visualizing molecular events at the cellular, tissue and whole‐body levels. Transmembrane transporters recognizing specific molecular domains are of particular interest because they enable the migration of signal‐source molecules from the extracellular space to the cytoplasm for subsequent application in multimodality imaging. Organic anion‐transporting polypeptides (OATPs) have demonstrated their MRI reporter efficacy. We further expanded their use as a dual‐modality reporter in MRI and noninvasive in vivo imaging system (IVIS). We overexpressed OATP1B3 in the HT‐1080 sarcoma cell line. Both Gd‐EOB‐DTPA, an MRI contrast agent, and indocyanine green (ICG), a near‐infrared fluorescent dye that provides better deep‐tissue detection because of its long wavelength, could be delivered to the intracellular space and imaged in a tumor‐bearing nude mouse model. Our in vivo dual‐imaging reporter system achieved high sensitivity in MRI and observation periods lasting as long as 96 h in IVIS. Because of the superior temporal and spatial resolutions and the clinical availability of both ICG and Gd‐EOB‐DTPA, this dual‐imaging OATP1B3 system will find biomedical use in tumor biology, stem cell trafficking, and tissue engineering.—Wu, M.‐R., Liu, H.‐M., Lu, C.‐W., Shen, W.‐H., Lin, I.‐J., Liao, L.‐W., Huang, Y.‐Y., Shieh, M.‐J., Hsiao, J.‐K. Organic anion‐transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imaging. FASEB J. 32,1705‐1715 (2018). www.fasebj.org