Premium
Quantitative high‐throughput screening identifies cytoprotective molecules that enhance SUMO conjugation via the inhibition of SUMO‐specific protease (SENP)2
Author(s) -
Bernstock Joshua D.,
Ye Daniel,
Smith Jayden A.,
Lee YangJa,
Gessler Florian A.,
Yasgar Adam,
Kouznetsova Jennifer,
Jadhav Ajit,
Wang Zhuoran,
Pluchino Stefano,
Zheng Wei,
Simeonov Anton,
Hallenbeck John M.,
Yang Wei
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201700711r
Subject(s) - sumo protein , neuroprotection , protease , proteases , microbiology and biotechnology , ubiquitin , small molecule , chemistry , biology , computational biology , neuroscience , biochemistry , enzyme , gene
The development of novel neuroprotective treatments for acute stroke has been fraught with failures, which supports the view of ischemic brain damage as a highly complex multifactorial process. Post‐translational modifications such as small ubiquitin‐like modifier (SUMO)ylation have emerged as critical molecular regulatory mechanisms in states of both homeostasis and ischemic stress, as evidenced by our previous work. Accordingly, the clinical significance of the selective control of the global SUMOylation process has become apparent in studies of ischemic pathobiology and pathophysiology. Herein, we describe a process capable of identifying and characterizing small molecules with the potential of targeting the SUMO system through inhibition of SUMO deconjugation in an effort to develop novel stroke therapies.—Bernstock, J. D., Ye, D., Smith, J. A., Lee, Y.‐J., Gessler, F. A., Yasgar, A., Kouznetsova, J., Jadhav, A., Wang, Z., Pluchino, S., Zheng, W., Simeonov, A., Hallenbeck, J. M., Yang, W. Quantitative high‐throughput screening identifies cytoprotective molecules that enhance SUMO‐conjugation via the inhibition of SUMO‐specific protease (SENP)2. FASEB J. 32, 1677‐1691 (2018). www.fasebj.org