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Effects of hawthorn ( Crataegus pentagyna ) leaf extract on electrophysiologic properties of cardiomyocytes derived from human cardiac arrhythmia‐specific induced pluripotent stem cells
Author(s) -
Pahlavan Sara,
Tousi Marziyeh Shalchi,
Ayyari Mahdi,
Alirezalu Abolfazl,
Ansari Hassan,
Saric Tomo,
Baharvand Hossein
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201700494rr
Subject(s) - induced pluripotent stem cell , pharmacology , medicine , stem cell , embryonic stem cell , traditional medicine , biology , microbiology and biotechnology , biochemistry , gene
Cardiac arrhythmias are major life‐threatening conditions. The landmark discovery of induced pluripotent stem cells has provided a promising in vitro system for modeling hereditary cardiac arrhythmias as well as drug development and toxicity testing. Nowadays, nutraceuticals are frequently used as supplements for cardiovascular therapy. Here we studied the cardiac effects of hawthorn ( Crataegus pentagyna ) leaf extract using cardiomyocytes (CMs) differentiated from healthy human embryonic stem cells, long QT syndrome type 2 (LQTS2), and catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) patient‐specific induced pluripotent stem cells. The hydroalcoholic extract resulted in a dose‐dependent negative chronotropic effect in all CM preparations leading to a significant reduction at 1000 μ g/ml. This was accompanied by prolongation of field potential durations, although with different magnitudes in CMs from different human embryonic stem cell and iPSC lines. Hawthorn further prolonged field potential durations in LQTS2 CMs but reduced the beating frequencies and occurrence of immature field potentials triggered by β 1 ‐adrenergic stimulation in CPVT1 CMs at 300 and 1000 μ g/ml. Furthermore, isoquercetin and vitexin flavonoids significantly slowed down isoproterenol (5 μ M)‐induced beating frequencies at 3 and 10 μg/ml. Therefore, C. pentagyna leaf extract and its isoquercetin and vitexin flavonoids may be introduced as a novel nutraceutical with antiarrhythmic potential for CPVT1 patients.—Pahlavan, S., Tousi, M. S., Ayyari, M., Alirezalu, A., Ansari, H., Saric, T., Baharvand, H. Effects of hawthorn ( Crataegus pentagyna ) leaf extract on electrophysiologic properties of cardiomyocytes derived from human cardiac arrhythmia‐specific induced pluripotent stem cells. FASEB J. 32,1440‐1451 (2018). www.fasebj.org