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Maternal Western diet age‐specifically alters female offspring voluntary physical activity and dopamine‐ and leptin‐related gene expression
Author(s) -
Ruegsegger Gregory N.,
Grigsby Kolter B.,
Kelty Taylor J.,
Zidon Terese M.,
Childs Thomas E.,
VieiraPotter Victoria J.,
Klinkebiel David L.,
Matheny Michael,
Scarpace Phillip J.,
Booth Frank W.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201700389r
Subject(s) - offspring , endocrinology , medicine , leptin , ventral tegmental area , biology , nucleus accumbens , leptin receptor , in utero , overnutrition , obesity , pregnancy , dopamine , fetus , dopaminergic , genetics
Prenatal overnutrition affects development into adulthood and influences risk of obesity. We assessed the transgenerational effect of maternal Western diet (WD) consumption on offspring physical activity. Voluntary wheel running was increased in juvenile (4–7 wk ofage), but decreased in adult (16–19 wk of age), F 1 female WD offspring. In contrast, no wheel‐running differences in F 1 male offspring were observed. Increased wheel running in juvenile female WD offspring was associated with up‐regulated dopamine receptor (DRD)‐1 and ‐2 in the nucleus accumbens (NAc) and with down‐regulated Lepr in the ventral tegmental area (VTA). Conversely, decreased wheel running by adult female WD offspring was associated with down‐regulated DRD1 in the NAc and with up‐regulated Lepr in the VTA. Body fat, leptin, and insulin were increased in male, but not in female, F 1 WD offspring. Recombinant virus (rAAV) leptin antagonism in the VTA decreased wheel running in standard diet but not in WD F 1 female offspring. Analysis of F 2 offspring found no differences in wheel running or adiposity in male or female offspring, suggesting that changes in the F 1 generation were related to in utero somatic reprogramming. Our findings indicate prenatal WD exposure leads to age‐specific changes in voluntary physical activity in female offspring that are differentially influenced by VTA leptin antagonism.—Ruegsegger, G. N., Grigsby, K. B., Kelty, T. J., Zidon, T. M., Childs, T. E., Vieira‐Potter, V. J., Klinkebiel, D. L., Matheny, M., Scarpace, P. J., Booth, F. W. Maternal Western diet age‐specifically alters female offspring voluntary physical activity and dopamine‐ and leptin‐related gene expression. FASEB J. 31, 5371–5383 (2017). www.fasebj.org