Premium
Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice
Author(s) -
Chatel Benjamin,
Bendahan David,
Hourdé Christophe,
Pellerin Luc,
Lengacher Sylvain,
Magistretti Pierre,
Le Fur Yann,
Vilmen Christophe,
Bernard Monique,
Messonnier Laurent A.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201601259r
Subject(s) - chemistry , skeletal muscle , energetics , in vivo , function (biology) , homeostasis , haploinsufficiency , microbiology and biotechnology , biophysics , biochemistry , endocrinology , biology , genetics , phenotype , ecology , gene
The purpose of this study was to investigate the effects of a partial suppression of monocarboxylate transporter (MCT)‐1 on skeletal muscle pH, energetics, and function (MCT1 +/ − mice). Twenty‐four MCT1 +/ − and 13 wild‐type (WT) mice were subjected to a rest–exercise–recovery protocol, allowing assessment of muscle energetics (by magnetic resonance spectroscopy) and function. The study included analysis of enzyme activities and content of protein involved in pH regulation. Skeletal muscle of MCT1 +/ − mice had lower MCT1 (‐61%; P < 0.05) and carbonic anhydrase (CA)‐II (‐54%; P < 0.05) contents. Although intramuscular pH was higher in MCT1 +/ − mice at rest ( P < 0.001), the mice showed higher acidosis during the first minute of exercise ( P < 0.01). Then, the pH time course was similar among groups until exercise completion. MCT1 +/ − mice had higher specific peak ( P < 0.05) and maximum tetanic ( P < 0.01) forces and lower fatigability ( P < 0.001) when compared to WT mice. We conclude that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1 +/ − mice remain unexplained.—Chatel, B., Bendahan, D., Hourdé, C., Pellerin, L., Lengacher, S., Magistretti, P., Fur, Y. L., Vilmen, C., Bernard, M., Messonnier, L. A. Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice. FASEB J. 31, 2562–2575 (2017). www.fasebj.org