Premium
Mitochondrial and redox abnormalities in autism lymphoblastoid cells: a sibling control study
Author(s) -
Rose Shan,
Bennuri Sirish C.,
Wynne Rebecca,
Melnyk Stepan,
James S. Jill,
Frye Richard E.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201601004r
Subject(s) - mitochondrion , biology , mitochondrial ros , glutathione , oxidative stress , bioenergetics , reactive oxygen species , mitochondrial disease , autism , oxidative phosphorylation , intracellular , redox , glycolysis , microbiology and biotechnology , biochemistry , mitochondrial dna , metabolism , gene , chemistry , medicine , psychiatry , enzyme , organic chemistry
Autism spectrum disorder (ASD) is associated with physiological abnormalities, including abnormal redox and mitochondrial metabolism. Lymphoblastoid cell lines (LCLs) from some children with ASD exhibit increased oxidative stress, decreased glutathione redox capacity, and highly active mitochondria with increased vulnerability to reactive oxygen species (ROS). Because unaffected siblings (Sibs) of individuals with ASD share some redox abnormalities, we sought to determine whether LCLs from Sibs share ASD‐associated mitochondrial abnormalities. We evaluated mitochondrial bioenergetics in 10 sets of LCLs from children with ASD, Sibs, and unrelated/unaffected controls (Cons) after acute increases in ROS. Additionally, intracellular glutathione and uncoupling protein 2 ( UCP 2) gene expressions were quantified. Compared to Sib LCLs, ASD LCLs exhibited significantly higher ATP‐linked respiration, higher maximal and reserve respiratory capacity, and greater glycolysis and glycolytic reserve. ASD LCLs exhibited a significantly greater change in these parameters, with acute increases in ROS compared to both Sib and Con LCLs. Compared to Con, both ASD and Sib LCLs exhibited significantly higher proton leak respiration. Consistent with this, intracellular glutathione redox capacity was decreased and UCP 2 gene expression was increased in both ASD and Sib compared to Con LCLs. These data indicate that mitochondrial respiratory function, not abnormal redox homeostasis, distinguishes ASD from unaffected LCLs.—Rose, S., Bennuri, S. C., Wynne, R., Melnyk, S., James, S. J., Frye, R. E. Mitochondrial and redox abnormalities in autism lymphoblastoid cells: a sibling control study. FASEB J. 31, 904–909 (2017). www.fasebj.org