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Lipoxin A 4 stimulates endothelial miR‐126–5p expression and its transfer via microvesicles
Author(s) -
Codag Marilina,
Recchiuti Antonio,
Lanuti Paola,
Pierdomenico Anna Maria,
Cianci Eleonora,
Patruno Sara,
Mari Veronica Cecilia,
Simiele Felice,
Di Tomo Pamela,
Pandolfi Assunta,
Romano Mario
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201600952r
Subject(s) - microvesicles , microvesicle , microbiology and biotechnology , chemistry , microrna , downregulation and upregulation , inflammation , transcellular , biochemistry , biology , immunology , gene
ABSTRACT The proresolution lipid mediator lipoxin (LX)A 4 bestows protective bioactions on endothelial cells. We examined the impact of LXA 4 on transcellular endothelial signaling via microRNA (miR)‐containing microvesicles. We report LXA 4 inhibition of MV release by TNF‐α‐treated HUVECs, associated with the down‐regulation of 18 miR in endothelial microvesicles (EMVs) and the up‐regulation of miR‐126–5p, both in HUVECs and in EMVs. LXA 4 upregulated miR‐126–5p by ∼5‐fold in HUVECs and promoted a release of microvesicles (LXA 4 ‐EMVs) that enhanced miR‐126–5p by ∼7‐fold in recipient HUVECs. In these cells, LXA 4 ‐EMVs abrogated the up‐regulation of VCAM‐1, induced in recipient HUVECs by EMVs released by untreated or TNF‐α‐treated HUVECs. LXA 4 ‐EMVs also reduced by ∼40% the expression of SPRED1, which we validated as an miR‐126–5p target, whereas they stimulated monolayer repair in an in vitro wound assay. This effect was lost when the EMVs were depleted of miR‐126–5p. These results provide evidence that changes in miR expression and microvesicle packaging and transfer represent a mechanism of action of LXA 4 , which may be relevant in vascular biology and inflammation.—Codagnone, M., Recchiuti, A., Lanuti, P., Pierdomenico, A. M., Cianci, E., Patruno, S., Mari, V. C., Simiele, F., Di Tomo, P., Pandolfi, A., Romano, M. Lipoxin A4 stimulates endothelial miR‐126–5p expression and its transfer via microvesicles. FASEB J. 31, 1856–1866 (2017). www.fasebj.org