Premium
Serum amyloid A: an ozone‐induced circulating factor with potentially important functions in the lung‐brain axis
Author(s) -
Erickson Michelle A.,
Jude Joseph,
Zhao Hengjiang,
Rhea Elizabeth M.,
Salameh Therese S.,
Jester William,
Pu Shelley,
Harrowitz Jenna,
Nguyen Ngan,
Banks William A.,
Panettieri Reynold A.,
JordanSciutto Kelly L.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201600857rrr
Subject(s) - ozone , lung , amyloid (mycology) , medicine , neuroscience , pathology , chemistry , psychology , organic chemistry
Accumulating evidence suggests that O 3 exposure may contribute to CNS dysfunction. Here, we posit that inflammatory and acute‐phase proteins in the circulation increase after O 3 exposure and systemically convey signals of O 3 exposure to the CNS. To model acute O 3 exposure, female Balb/c mice were exposed to 3 ppm O 3 or forced air for 2 h and were studied after 6 or 24 h. Of 23 cytokines and chemokines, only KC/CXCL1 was increased in blood 6 h after O 3 exposure. The acute‐phase protein serum amyloid A (A‐SAA) was significantly increased by 24 h, whereas C‐reactive protein was unchanged. A‐SAA in blood correlated with total leukocytes, macrophages, and neutrophils in bronchoalveolar lavage from O 3 ‐exposed mice. A‐SAA mRNA and protein were increased in the liver. We found that both isoforms of A‐SAA completely crossed the intact blood‐brain barrier, although the rate of SAA2.1 influx was approximately 5 times faster than that of SAA1.1. Finally, A‐SAA protein, but not mRNA, was increased in the CNS 24 h post‐O 3 exposure. Our findings suggest that A‐SAA is functionally linked to pulmonary inflammation in our O 3 exposure model and that A‐SAA could be an important systemic signal of O 3 exposure to the CNS.—Erickson, M. A., Jude, J., Zhao, H., Rhea, E. M., Salameh, T. S., Jester, W., Pu, S., Harrowitz, J., Nguyen, N., Banks, W. A., Panettieri, R. A., Jr., Jordan‐Sciutto, K. L. Serum amyloid A: an ozone‐induced circulating factor with potentially important functions in the lung‐brain axis. FASEB J. 31, 3950–3965 (2017). www.fasebj.org —Erickson, Michelle A., Jude, Joseph, Zhao, Hengjiang, Rhea, Elizabeth M., Salameh, Therese S., Jester, William, Pu, Shelley, Harrowitz, Jenna, Nguyen, Ngan, Banks, William A., PanettieriJr., Reynold A., Jordan‐Sciutto, Kelly L. Serum amyloid A: an ozone‐induced circulating factor with potentially important functions in the lung‐brain axis. FASEB J. 31, 3950–3965 (2017)