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Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish
Author(s) -
Martella Andrea,
Sepe Rosa M.,
Silvestri Cristoforo,
Zang Jingjing,
Fasano Giulia,
Carnevali Oliana,
De Girolamo Paolo,
Neuhauss Stephan C. F.,
Sordino Paolo,
Di Marzo Vincenzo
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201600602r
Subject(s) - endocannabinoid system , zebrafish , cannabinoid receptor , 2 arachidonoylglycerol , monoacylglycerol lipase , neuroscience , biology , microbiology and biotechnology , anabolism , fatty acid amide hydrolase , receptor , endocrinology , gene , biochemistry , agonist
The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report thepresenceof a complete endocannabinoid system during zebrafish development andshowthat thegenes that code for enzymes that catalyze the anabolism and catabolism ( mgll and dagla ) of the endocannabinoid, 2‐AG (2‐arachidonoylglycerol), as well as 2‐AGmain receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino‐induced transient knockdown of the zebrafish Daglα homologandits pharmacologic rescue, we suggest that synthesis of 2‐AGis implicated inthe control of axon formationin themidbrain‐hindbrain region andthat animals that lack Dagl α display abnormal physiological behaviors intests that measure stereotypedmovement andmotion perception. Our results suggest that thewell‐established role for 2‐AG in axonal outgrowth has implications for the control of vision andmovement in zebrafish and, thus, is likely common to all vertebrates.—Martella, A., Sepe, R.M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S.C. F., Sordino, P., Di Marzo, V. Important role of endocannabinoid signaling inthe development of functional vision and locomotion in zebrafish. FASEB J. 30, 4275–4288 (2016). www.fasebj.org