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Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity
Author(s) -
CarrascoBenso Maria P.,
RiveroGutierrez Belen,
LopezMinguez Jesus,
Anzola Andrea,
DiezNoguera Antoni,
Madrid Juan A.,
Lujan Juan A.,
MartínezAugustin Olga,
J. L. Scheer Frank A.,
Garaulet Marta
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201600269rr
Subject(s) - circadian rhythm , adipose tissue , insulin sensitivity , medicine , endocrinology , rhythm , insulin , insulin resistance
In humans, insulin sensitivity varies according to time of day, with decreased values in the evening and at night. Mechanisms responsible for the diurnal variation in insulin sensitivity are unclear. We investigated whether human adipose tissue (AT) expresses intrinsic circadian rhythms in insulin sensitivity that could contribute to this phenomenon. Subcutaneous and visceral AT biopsies were obtained from extremely obese participants (body mass index, 41.8 ± 6.3 kg/m 2 ;46 ± 11 y) during gastric‐bypass surgery. To assess the rhythm in insulin signaling, AKT phosphorylation was determined every 4 h over 24 h in vitro in response to different insulin concentrations (0, 1, 10, and 100 nM). Data revealed that subcutaneous AT exhibited robust circadian rhythms in insulin signaling ( P <0.00001). Insulin sensitivity reached its maximum (acrophase) around noon, being 54% higher than during midnight ( P = 0.009). The amplitude of the rhythm was positively correlated with in vivo sleep duration (r =0.53; P =0.023) and negatively correlated with in vivo bedtime (r = 20.54; P = 0.020). No circadian rhythms were detected in visceral AT ( P = 0.643). Here, we demonstrate the relevance of the time of the day for how sensitive AT is to the effects of insulin. Subcutaneous AT shows an endogenous circadian rhythm in insulin sensitivity that could provide an underlying mechanism for the daily rhythm in systemic insulin sensitivity.—Carrasco‐Benso, M. P., Rivero‐Gutierrez, B., Lopez‐Minguez, J., Anzola, A., Diez‐Noguera, A., Madrid, J.A., Lujan, J.A., Martínez‐Augustin, O., Scheer, F.A. J.L., Garaulet, M. Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. FASEB J. 30, 3117–3123 (2016). www.fasebj.org