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Cold/menthol TRPM8 receptors initiate the cold‐shock response and protect germ cells from cold‐shock–induced oxidation
Author(s) -
Borowiec AnneSophie,
Sion Benoit,
Chalmel Fréric,
Rolland Antoine D.,
Lemonnier Loïc,
De Clerck Tatiana,
Bokhobza Alexandre,
Derouiche Sandra,
Dewailly Etienne,
Slomianny Christian,
Mauduit Claire,
Benahmed Mohamed,
Roudbaraki Morad,
Jégou Bernard,
Prevarskaya Natalia,
Bidaux Gabriel
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201600257r
Subject(s) - trpm8 , cold shock domain , microbiology and biotechnology , receptor , knockout mouse , apoptosis , biology , cold storage , chemistry , transient receptor potential channel , biochemistry , gene , rna , trpv1 , horticulture
Testes of most male mammals present the particularity of being externalized from the body and are consequently slightly cooler than core body temperature (4–8°C below). Although, hypothermia of the testis is known to increase germ cells apoptosis, little is known about the underlying molecular mechanisms, including cold sensors, transduction pathways, and apoptosis triggers. In this study, using a functional knockout mouse model of the cold and menthol receptors, dubbed transient receptor potential melastatine 8 (TRPM8) channels, we found that TRPM8 initiated the cold‐shock response by differentially modulating cold‐and heat‐shock proteins. Besides, apoptosis of germ cells increased in proportion to the cooling level in control mice but was independent of temperature in knockout mice. We also observed that the rate of germ cell death correlated positively with the reactive oxygen species level and negatively with the expression of the detoxifying enzymes. This result suggests that the TRPM8 sensor is a key determinant of germ cell fate under hypothermic stimulation.—Borowiec, A.‐S., Sion, B., Chalmel, F., Rolland, A.D., Lemonnier, L., De Clerck, T., Bokhobza, A., Derouiche, S., Dewailly, E., Slomianny, C., Mauduit, C., Benahmed, M., Roudbaraki, M., Jégou, B., Prevarskaya, N., Bidaux, G. Cold/menthol TRPM8 receptors initiate the cold‐shock response and protect germ cells from cold‐shock–induced oxidation. FASEBJ. 30, 3155–3170 (2016). www.fasebj.org