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Improvement in adiposity with oligofructose is modified by antibiotics in obese rats
Author(s) -
Bomhof Marc R.,
Paul Heather A.,
Geuking Markus B.,
Eller Lindsay K.,
Reimer Raylene A.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201600151r
Subject(s) - ampicillin , neomycin , gut flora , prebiotic , antibiotics , lactobacillus , biology , food science , probiotic , chemistry , microbiology and biotechnology , bacteria , biochemistry , fermentation , genetics
Given the intimate link between gut microbiota and host physiology, there is growing interest in understanding the mechanisms by which diet influences gut microbiota and affects human metabolic health. Using antibiotics and the prebiotic oligofructose, which has been shown to counteract excess fat mass, we explored the gut microbiota‐dependent effects of oligofructose on body composition and host metabolism. Diet‐induced obese male Sprague Dawley rats, fed a background high‐fat/sucrose diet, were randomized to one of the following diets for 6 wk: 1) high‐energy control; 2) 10% oligofructose; 3) ampicillin; 4) ampicillin + 10% oligofructose; 5) ampicillin/neomycin; or 6) ampicillin/neomycin + 10% oligofructose. Combining oligofructose with ampicillin treatment blunted the decrease in adiposity seen with oligofructose. Although ampicillin did not affect total bacteria, ampicillin impeded oligofructose‐induced increases in Bifidobacterium and Lactobacillus. In contrast, the combination of ampicillin and neomycin reduced total bacteria but did not abrogate the oligofructose‐induced decrease in adiposity. Oligofructose‐mediated effects on host adiposity and metabolic health appear to be in part dependent on the presence of specific microbial species within the gut.—Bomhof, M. R., Paul, H. A., Geuking, M. B., Eller, L. K., Reimer, R. A. Improvement in adiposity with oligofructose is modified by antibiotics in obese rats. FASEB J. 30, 2720‐2732 (2016). www.fasebj.org