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Lipoxin A 4 augments host defense in sepsis and reduces Pseudomonas aeruginosa virulence through quorum sensing inhibition
Author(s) -
Wu Benedict,
Capilato Joseph,
Pham Michelle P.,
Walker Jean,
Spur Bernd,
Rodriguez Ana,
Perez Lark J.,
Yin Kingsley
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.201500029r
Subject(s) - quorum sensing , pseudomonas aeruginosa , virulence , host (biology) , microbiology and biotechnology , sepsis , biology , bacteria , immunology , genetics , gene
Bacterial infections can quickly turn into sepsis, with its attendant clinical sequelae of inflammation, tissue injury, and organ failure. Paradoxically, sustained inflammation in sepsis may lead to immune suppression, because of which the host is unable to clear the existing infection. Use of agents that suppress the inflammatory response may accelerate host immune suppression, whereas use of traditional antibiotics does not significantly affect inflammation. In this study, we investigated whether lipoxin A 4 (LXA 4 ), a specialized, proresolution lipid mediator, could increase neutrophil phagocytic activity as well as reduce bacterial virulence. Using the mouse cecal ligation and puncture (CLP) model of sepsis, the administration of LXA 4 (7 μg/kg i.v.) 1 h after surgery increased neutrophil phagocytic ability and Fcg receptor I (CD64) expression. Ex vivo studies have confirmed that the direct addition of LXA 4 to CLP neutrophils increased phagocytic ability but not CD64 expression. LXA 4 did not affect neutrophils taken from control mice in which CD64 expression was minimal. Taken together with in vivo data, these results suggest that LXA 4 directly augments CD64‐mediated neutrophil phagocytic ability but does not directly increase neutrophil CD64 expression. Bacterial communication and virulence is regulated by quorum sensing inducers. In Pseudomonas aeruginosa , virulence is induced with release of various virulence factors, by N ‐3‐oxododecanolyl homoserine lactone binding to the quorum sensing receptor, LasR. We show that LXA 4 is an inhibitor of LasR in P. aeruginosa and that it decreases the release of pyocyanin exotoxin. These results suggest that LXA 4 has the novel dual properties of increasing host defense and decreasing pathogen virulence by inhibiting quorum sensing.—Wu, B., Capilato, J., Pham, M. P., Walker, J., Spur, B., Rodriguez, A., Perez, L. J., Yin, K. Lipoxin A 4 augments host defense in sepsis and reduces Pseudomonas aeruginosa virulence through quorum sensing inhibition. FASEB J. 30, 2400–2410 (2016). www.fasebj.org