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The VP3 structural protein of foot‐and‐mouth disease virus inhibits the IFN‐β signaling pathway
Author(s) -
Li Dan,
Yang Wenping,
Yang Fan,
Liu Huanan,
Zhu Zixiang,
Lian Kaiqi,
Lei Caoqi,
Li Shu,
Liu Xiangtao,
Zheng Haixue,
Shu Hongbing
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.15-281410
Subject(s) - foot and mouth disease virus , sendai virus , innate immune system , virus , signal transduction , signal transducing adaptor protein , biology , virology , transfection , scaffold protein , microbiology and biotechnology , immune system , gene , immunology , biochemistry
Foot‐and‐mouth disease is a frequently occurring disease of cloven‐hoofed animals that is caused by infection with the foot‐and‐mouth virus (FMDV). FMDV circumvents the type‐I IFN response by expressing proteins that antagonize cellular innate immunity, such as leader protease and 3C protease. We identified the FMDV structural protein VP3 as a negative regulator of the virus‐triggered IFN‐β signaling pathway. Expression of FMDV VP3 inhibited the Sendai virus‐triggered activation of IFN regulatory factor‐3 and the expression of retinoic acid‐inducible gene‐I/melanoma differentiation‐associated protein‐5. Transient transfection and coimmunoprecipitation confirmed that the structural protein VP3 interacts with virus‐induced signaling adapter (VISA), which is dependent on the C‐terminal aa 111–220 of VP3. In addition, we found that FMDV VP3 inhibits the expression of VISA by disrupting its mRNA. Taken together, our findings reveal a novel strategy used by the structural VP3 protein of FMDV to evade host innate immunity.—Li, D., Yang, W., Yang, F., Liu, H., Zhu, Z., Lian, K., Lei, C., Li, S., Liu, X., Zheng, H., Shu, H. The VP3 structural protein of foot‐and‐mouth disease virus inhibits the IFN‐β signaling pathway. FASEB J. 30, 1757–1766 (2016). www.fasebj.org