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An agonist antibody that blocks autoimmunity by inducing anti‐inflammatory macrophages
Author(s) -
Han Kyung Ho,
GonzalezQuintial Rosana,
Peng Yingjie,
Baccala Roberto,
Theofilopoulos Argyrios N.,
Lerner Richard A.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.15-281329
Subject(s) - autoimmunity , agonist , antibody , immunology , macrophage , inflammation , biology , receptor , in vitro , genetics
We have devised a method of using intracellular combinatorial libraries to select antibodies that control cell fates. Many agonist antibodies have been selected with this method, and the process appears to be limited only by the availability of a phenotypic selection system. We demonstrate the utility of this approach to discover agonist antibodies that engage an unanticipated target and regulate macrophage polarization by selective induction of anti‐inflammatory M2 macrophages. This antibody was used therapeutically to block autoimmunity in a classic mouse model of spontaneous systemic lupus erythematosus.—Han, K. H., Gonzalez‐Quintial, R., Peng, Y., Baccala R., Theofilopoulos, A. N., Lerner, R. A. An agonist antibody that blocks autoimmunity by inducing anti‐inflammatory macrophages. FASEB J. 30, 738‐747 (2016). www.fasebj.org