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De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue
Author(s) -
Gavin Kathleen M.,
Gutman Jonathan A.,
Kohrt Wendy M.,
Wei Qi,
Shea Karen L.,
Miller Heidi L.,
Sullivan Timothy M.,
Erickson Paul F.,
Helm Karen M.,
Acosta Alistaire S.,
Childs Christine R.,
Musselwhite Evelyn,
VarellaGarcia Marileila,
Kelly Kimberly,
Majka Susan M.,
Klemm Dwight J.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.15-278994
Subject(s) - adipose tissue , adipocyte , progenitor cell , bone marrow , biology , stromal cell , mesenchymal stem cell , haematopoiesis , stem cell , microbiology and biotechnology , immunology , endocrinology , cancer research
White adipocytes in adults are typically derived from tissue resident mesenchymal progenitors. The recent identification of de novo production of adipocytes from bone marrow progenitor‐derived cells in mice challenges this paradigm and indicates an alternative lineage specification that adipocytes exist. We hypothesized that alternative lineage specification of white adipocytes is also present in human adipose tissue. Bone marrow from transgenic mice in which luciferase expression is governed by the adipocyte‐restricted adiponectin gene promoter was adoptively transferred to wild‐type recipient mice. Light emission was quantitated in recipients by in vivo imaging and direct enzyme assay. Adipocytes were also obtained from human recipients of hematopoietic stem cell transplantation. DNA was isolated, and microsatellite polymorphisms were exploited to quantify donor/recipient chimerism. Luciferase emission was detected from major fat depots of transplanted mice. No light emission was observed from intestines, liver, or lungs. Up to 35% of adipocytes in humans were generated from donor marrow cells in the absence of cell fusion. Nontransplanted mice and stromal‐vascular fraction samples were used as negative and positive controls for the mouse and human experiments, respectively. This study provides evidence for a nontissue resident origin of an adipocyte subpopulation in both mice and humans.—Gavin, K. M., Gutman, J. A., Kohrt, W. M., Wei, Q., Shea, K. L., Miller, H. L., Sullivan, T. M., Erickson, P. F., Helm, K. M., Acosta, A. S., Childs, C. R., Musselwhite, E., Varella‐Garcia, M., Kelly, K., Majka, S. M., Klemm, D. J., De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue. FASEB J. 30, 1096–1108 (2016). www.fasebj.org