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Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox‐sensitive genes
Author(s) -
Wang Wei,
Degroote Jeroen,
Van Ginneken Chris,
Van Poucke Mario,
Vergauwen Hans,
Dam Thi Minh Tho,
Vanrompay Daisy,
Peelman Luc J.,
De Smet Stefaan,
Michiels Joris
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.15-274779
Subject(s) - intrauterine growth restriction , malondialdehyde , intestinal permeability , endocrinology , medicine , glutathione , glutathione peroxidase , andrology , catalase , chemistry , small intestine , occludin , barrier function , biology , oxidative stress , biochemistry , fetus , tight junction , enzyme , pregnancy , microbiology and biotechnology , genetics
Neonates with intrauterine growth restriction (IUGR) show lower efficiency of nutrient utilization compared to normal birth weight (NBW) newborns. This study was conducted using neonatal piglets as a model to test the hypothesis that IUGR affects the intestinal barrier function, intestinal structure, and antioxidant system development during the suckling period. The small intestinal mucosae were obtained from IUGR and NBW littermates in the suckling period (d 0, 3, 8, and 19 postnatal). The epithelial barrier function was assessed by FITC‐dextran 4 (FD4) and horseradish peroxidase (HRP) fluxes across the epithelium, histomorphologic measurements, and expression of tight‐junction proteins. Redox status represented by the glutathione disulfide/glutathione ratio and malondialdehyde concentrations was determined, whereas mRNA expressions of some redox‐sensitive proteins were quantified. Results showed that IUGR piglets exhibited a 2‐fold higher intestinal permeability in the proximal small intestine on d 0 ( P < 0.05), and this difference between IUGR and NBW piglets was widened to 3 and 4 times for FD4 and HRP, respectively ( P < 0.05), on d 3. In accordance, expression of occludin was downregulated at the transcriptional level in IUGR piglets at d 0 and 19 ( P < 0.01). Furthermore, the transcription of heme oxygenase 1, catalase , and thioredoxin reductase genes was downregulated in IUGR piglets, mainly on postnatal d 0 and 19 ( P < 0.01). It appears that IUGR subjects have a lower capacity to mount an antioxidant response in the early postnatal period. Collectively, these results add to our understanding of the mechanisms responsible for intestinal dysfunction in IUGR neonates.—Wang, W., Degroote, J., Van Ginneken, C., Van Poucke, M., Vergauwen, H., Dam, T. M. T., Vanrompay, D., Peelman, L. J., De Smet, S., Michiels, J. Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox‐sensitive genes. FASEB J. 30, 863–873 (2016). www.fasebj.org