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Combination of small RNAs for skeletal muscle regeneration
Author(s) -
Kim NaJung,
Yoo James J.,
Atala Anthony,
Lee Sang Jin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.15-271809
Subject(s) - regeneration (biology) , rna interference , skeletal muscle , microbiology and biotechnology , microrna , myostatin , biology , small interfering rna , myocyte , muscle atrophy , myogenesis , gene expression , rna , gene , anatomy , genetics
Selectively controlling the expression of the target genes through RNA interference (RNAi) has significant therapeutic potential for injuries or diseases of tissues. We used this strategy to accelerate and enhance skeletal muscle regeneration for the treatment of muscular atrophy. In this study, we used myostatin small interfering (si)RNA (siGDF‐8), a major inhibitory factor in the development and postnatal regeneration of skeletal muscle and muscle‐specific microRNAs (miR‐1 and ‐206) to further accelerate muscle regeneration. This combination of 3 small RNAs significantly improved the gene expression of myogenic regulatory factors in vitro , suggesting myogenic activation. Moreover, cell proliferation and myotube formation improved without compromising each other, which indicates the myogenic potential of this combination of small RNAs. The recovery of chemically injured tibialis anterior muscles in rats was significantly accelerated, both functionally and structurally. This novel combination of siRNA and miRNAs has promising therapeutic potential to improve in situ skeletal muscle regeneration.—Kim, N., Yoo, J. J., Atala, A., Lee, S. J., Combination of small RNAs for skeletal muscle regeneration. FASEB J. 30, 1198–1206 (2016). www.fasebj.org