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Neisseria meningitis GNA1030 is a ubiquinone‐8 binding protein
Author(s) -
Donnarumma Danilo,
Golfieri Giacomo,
Brier Sébastien,
Castagnini Marta,
Veggi Daniele,
Bottomley Matthew James,
Delany Isabel,
Norais Nathalie
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.14-263954
Subject(s) - neisseria , neisseria meningitidis , bacterial outer membrane , chemistry , biology , groes , biochemistry , gene , microbiology and biotechnology , escherichia coli , groel , bacteria , genetics
ABSTRACT Bexsero, a new vaccine against Neisseria meningitidis serogroup B (MenB), is composed of 3 main recombinant proteins and an outer membrane vesicle component. One of the main bactericidal antigens, neisseria heparin binding antigen (NHBA), is present as a fusion protein with the accessory protein genome‐derived neisserial antigen (GNA) 1030 to further increase its immunogenicity. The gene encoding for GNA1030 is present and highly conserved in all Neisseria strains, and although orthologs are present in numerous species, its biologic function is unknown. Native mass spectrometry was used to demonstrate that GNA1030 forms a homodimer associated with 2 molecules of ubiquinone‐8 (Ub8), a cofactor mainly involved in the electron transport chain and with antioxidant properties. Disc diffusion assays on the wild‐type and knockout mutant of GNA1030, in the presence of various compounds, suggested that GNA1030 is not involved in oxidative stress or electron chain transport per se , although it contributes to constitutive refilling of the inner membrane with Ub8. These studies shed light on an accessory protein present in Bexsero and reveal functional insights into the family of related proteins. On the basis of our findings, we propose to name the protein neisseria ubiquinone binding protein (NUbp).—Donnarumma, D., Golfieri, G., Brier, S., Castagnini, M., Veggi, D., Bottomley, M. J., Delany, I., Norais, N. Neisseria meningitis GNA1030 is a ubiquinone‐8 binding protein. FASEB J. 29, 2260‐2267 (2015). www.fasebj.org