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Circadian organization of the rodent retina involves strongly coupled, layer‐specific oscillators
Author(s) -
Jaeger Catherine,
Sandu Cristina,
Malan André,
Mellac Katell,
Hicks David,
FelderSchmittbuhl MariePaule
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.14-261214
Subject(s) - retina , per1 , circadian rhythm , period (music) , biology , circadian clock , neuroscience , endogeny , microbiology and biotechnology , retinal , vibratome , inner nuclear layer , per2 , clock , biophysics , endocrinology , central nervous system , biochemistry , physics , acoustics
Rhythmic physiology is central to retinal function and survival and adapts vision to daily light intensity changes. Mammalian retina rhythmically releases melatonin when cultured under constant conditions, and the occurrence of clock gene [ e.g. , Period( Per )] expression has been shown for most cellular layers. However, contribution of the distinct layers to genesis of circadian rhythms within the retina is still debated. To characterize their endogenous oscillatory capacity and their communication at the whole‐tissue level, we used a vibratome‐based method to isolate individual or paired retina cellular layers from the mPer2 Luc mouse and Per1 ‐luciferase ( Per1‐Luc ) rat, and real‐time recorded bioluminescence. We report that each layer of the mouse retina harbors a self‐sustained oscillator whose period is significantly longer (~26 hours) than in whole‐retina explants (~22.9 hours), indicating that the period is correlated with the degree of coupling. Accordingly, the maximal period (~29 hours) is reached upon complete enzymatic dissociation of the retina. By using pharmacological approaches, we demonstrate that connection between retina oscillators involves gap junctions but only minor contribution from the main retina neurochemicals. Taken together with results from Per1‐Luc rats, these data show that mammalian retina consists of a network of layer‐specific oscillators whose period is determined by their connectivity.—Jaeger, C., Sandu, C., Malan, A., Mellac, K., Hicks, D., Felder‐Schmittbuhl, M.‐P. Circadian organization of the rodent retina involves strongly coupled, layer‐specific oscillators. FASEB J. 29, 1493‐1504 (2015). www.fasebj.org