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Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging
Author(s) -
Lescale Chloe,
Schenten Veronique,
Djeghloul Dounia,
Bennabi Meriem,
Gaignier Fanny,
Vandamme Katleen,
Strazielle Catherine,
Kuzniak Isabelle,
Petite Herve,
Dosquet Christine,
Frippiat JeanPol,
Goodhardt Michele
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.14-259770
Subject(s) - lymphopoiesis , progenitor cell , haematopoiesis , spaceflight , bone marrow , b cell , hindlimb , myelopoiesis , biology , microbiology and biotechnology , immunology , anatomy , stem cell , aerospace engineering , antibody , engineering
Within the bone marrow, the endosteal niche plays a crucial role in B‐cell differentiation. Because spaceflight is associated with osteoporosis, we investigated whether changes in bone microstructure induced by a ground‐based model of spaceflight, hind limb unloading (HU), could affect B lymphopoiesis. To this end, we analyzed both bone parameters and the frequency of early hematopoietic precursors and cells of the B lineage after 3, 6, 13, and 21 d of HU. We found that limb disuse leads to a decrease in both bone microstructure and the frequency of B‐cell progenitors in the bone marrow. Although multi‐potent hematopoietic progenitors were not affected by HU, a decrease in B lymphopoiesis was observed as of the common lymphoid progenitor (CLP) stage with a major block at the progenitor B (pro‐B) to precursor B (pre‐B) cell transition (5‐ to 10‐fold decrease). The modifications in B lymphopoiesis were similar to those observed in aged mice and, as with aging, decreased B‐cell generation in HU mice was associated with reduced expression of B‐cell transcription factors, early B‐cell factor (EBF) and Pax5, and an alteration in STAT5‐mediated IL‐7 signaling. These findings demonstrate that mechanical unloading of hind limbs results in a decrease in early B‐cell differentiation resembling age‐related modifications in B lymphopoiesis.—Lescale, C., Schenten, V., Djeghloul, D., Bennabi, M., Gaignier, F., Vandamme, K., Strazielle, C., Kuzniak, I., Petite, H., Dosquet, C., Frippiat, J.‐P., Goodhardt, M. Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging. FASEB J . 29, 455‐463 (2015). www.fasebj.org