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Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis
Author(s) -
Salanova Michele,
Gelfi Cecilia,
Moriggi Manuela,
Vasso Michele,
Viganò Agnese,
Minafra Luigi,
Bonifacio Gaetano,
Schiffl Gudrun,
Gutsmann Martina,
Felsenberg Dieter,
Cerretelli Paolo,
Blottner Dieter
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.14-252825
Subject(s) - desmin , myofibril , muscle atrophy , skeletal muscle , myofilament , atrophy , myocyte , cytoskeleton , biology , anatomy , medicine , microbiology and biotechnology , pathology , endocrinology , biochemistry , cell , immunohistochemistry , vimentin
In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower‐limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN‐C, ROCK1, and FAK); and expression of PGC1a and atrophy‐related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤0.01), WDR1 (39%, P ≤0.01), sarcosin (84%, P ≤0.01), and CKM (20%, P ≤0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN‐C increased (59%, P ≤0.01); the latter also with RE (108%, P ≤0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT‐slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultra‐structural and molecular dysregulation induced by chronic disuse.—Salanova, M., Gelfi, C., Moriggi, M., Vasso, M., Viganò, A., Minafra, L., Bonifacio, G., Schiffl, G., Gutsmann, M., Felsenberg, D., Cerretelli, P., Blottner, D., Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis. FASEB J. 28, 4748–4763 (2014). www.fasebj.org