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Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice
Author(s) -
Tani Yukako,
Isobe Yosuke,
Imoto Yuki,
SegiNishida Eri,
Sugimoto Yukihiko,
Arai Hiroyuki,
Arita Makoto
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.14-251132
Subject(s) - inflammation , cxcl13 , lymph , ccl11 , germinal center , lymph node , eosinophil , zymosan , immunology , medicine , chemokine , pathology , chemistry , eotaxin , chemokine receptor , b cell , antibody , asthma , biochemistry , in vitro
Resolution of inflammation is critical to restoration of tissue function after an inflammatory response. We previously demonstrated that 12/15‐lipoxygenase (12/15‐LOX)‐expressing eosinophils contribute to this process in murine zymosan‐induced peritonitis. In this study, eosinophils promoted resolution by regulating expression of macrophage CXCL13. Microarray analysis revealed that eosinophils significantly increased (~ 3‐fold) the expression of macrophage CXCL13 by a 12/15‐LOX‐dependent mechanism. CXCL13 depletion caused a resolution defect, with the reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph nodes. Inflamed lymph node hypertrophy, a critical feature of the resolution process, was reduced by ~60% in eosinophil‐deficient mice, and adoptive transfer of eosinophils or administration of CXCL13 corrected this defect. Administration of the 12/15‐LOX‐derived mediator lipoxin A 4 (LXA 4 ) increased the expression of CXCL13 and restored the defect of lymph node hypertrophy in eosinophil‐deficient mice. These results demonstrate that eosinophils control the resolution of inflammation and draining lymph node hypertrophy through proresolving lipid mediators and the CXCL13 pathway in mice.—Tani, Y., Isobe, Y., Imoto, Y., Segi‐Nishida, E., Sugimoto, Y., Arai, H., Arita, M. Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice. FASEB J. 28, 4036‐4043 (2014). www.fasebj.org