Premium
Simulated microgravity‐induced mitochondrial dysfunction in rat cerebral arteries
Author(s) -
Zhang Ran,
Ran HaiHong,
Cai LiLi,
Zhu Li,
Sun JunFang,
Peng Liang,
Liu XiaoJuan,
Zhang LanNing,
Fang Zhou,
Fan YongYan,
Cui Geng
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.13-245654
Subject(s) - apocynin , reactive oxygen species , mesenteric arteries , oxidative stress , mitochondrion , mitochondrial permeability transition pore , nadph oxidase , endocrinology , medicine , chemistry , mitochondrial ros , mptp , biology , biochemistry , artery , apoptosis , programmed cell death , dopamine , dopaminergic
Exposure to microgravity results in cardiovascular deconditioning, and cerebrovascular oxidative stress injury has been suggested to occur. To elucidate the mechanism for this condition, we investigated whether simulated microgravity induces mitochondrial dysfunction in rat arteries. Four‐week hindlimb unweighting (HU) was used to simulate microgravity in rats. Mitochondrial reactive oxygen species (ROS), mitochondrial membrane potential (Δψm), mitochondrial permeability transition pore (mPTP) opening, mitochondrial respiratory control ratio (RCR), MnSOD/GPx activity and expression, and mitochondrial malondialdehyde (MDA) were examined in rat cerebral and mesenteric VSMCs. Compared with the control rats, mitochondrial ROS levels, mPTP opening, and MDA content increased significantly ( P < 0.001, P <0.01, and P <0.01, respectively), Δψm, RCR, MnSOD/GPx activity ( P <0.001 for Δψm and RCR; P <0.05 for MnSOD; and P < 0.001 for GPx activity) and protein abundance of mitochondrial MnSOD/GPx‐1 decreased ( P <0.001 for MnSOD and GPx‐1) in HU rat cerebral but not mesenteric arteries. Chronic treatment with NADPH oxidase inhibitor apocynin and mitochondria‐targeted antioxidant mitoTempol promoted recovery of mitochondrial function in HU rat cerebral arteries, but exerted no effects on HU rat mesenteric arteries. Therefore, simulated microgravity resulted in cerebrovascular mitochondrial dysfunction, and crosstalk between NADPH oxidase and mitochondria participated in the process.—Zhang, R., Ran, H.‐H., Cai, L.‐L, Zhu, L., Sun, J.‐F., Peng, L., Liu, X.‐J., Zhang, L.‐N., Fang, Z., Fan, Y.‐Y., Cui, G. Simulated microgravity induced mitochondrial dysfunction in rat cerebral arteries. FASEB J . 28, 2715–2724 (2014). www.fasebj.org