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Regulation of the muscle fiber micro environment by activated satellite cells during hypertrophy
Author(s) -
Fry Christopher S.,
Lee Jonah D.,
Jackson Janna R.,
Kirby Tyler J.,
Stasko Shawn A.,
Liu Honglu,
DupontVersteegden Esther E.,
McCarthy John J.,
Peterson Charlotte A.
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.13-239426
Subject(s) - muscle hypertrophy , satellite , fiber , microbiology and biotechnology , medicine , chemistry , biology , physics , organic chemistry , astronomy
Our aim in the current study was to determine the necessity of satellite cells for long‐term muscle growth and maintenance. We utilized a transgenic Pax7‐DTA mouse model, allowing for the conditional depletion of > 90% of satellite cells with tamoxifen treatment. Synergist ablation surgery, where removal of synergist muscles places functional overload on the plantaris, was used to stimulate robust hypertrophy. Following 8 wk of overload, satellite cell‐depleted muscle demonstrated an accumulation of extracellular matrix (ECM) and fibroblast expansion that resulted in reduced specific force of the plantaris. Although the early growth response was normal, an attenuation of hypertrophy measured by both muscle wet weight and fiber cross‐sectional area occurred in satellite cell‐depleted muscle. Isolated primary myogenic progenitor cells (MPCs) negatively regulated fibroblast ECM mRNA expression in vitro , suggesting a novel role for activated satellite cells/MPCs in muscle adaptation. These results provide evidence that satellite cells regulate the muscle environment during growth.—Fry, C. S., Lee, J. D., Jackson, J. R., Kirby, T. J., Stasko, S. A, Liu, H., Dupont‐Versteegden, E. E., McCarthy, J. J., Peterson, C. A. Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. FASEB J. 28, 28–1654 (1665). www.fasebj.org