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Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy
Author(s) -
Ishikawa Keijiro,
Yoshida Shigeo,
Nakao Shintaro,
Nakama Takahito,
Kita Takeshi,
Asato Ryo,
Sassa Yukio,
Arita Ryoichi,
Miyazaki Masanori,
Enaida Hiroshi,
Oshima Yuji,
Murakami Noboru,
Niiro Hiroaki,
Ono Junya,
Matsuda Akira,
Goto Yoshinobu,
Akashi Koichi,
Izuhara Kenji,
Kudo Akira,
Kono Toshihiro,
HafeziMoghadam Ali,
Ishibashi Tatsuro
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.13-229740
Subject(s) - periostin , proliferative vitreoretinopathy , microbiology and biotechnology , retinal , chemistry , cell adhesion molecule , cancer research , adhesion , retinal detachment , medicine , biology , extracellular matrix , biochemistry , organic chemistry
Proliferative vitreoretinopathy (PVR) is a severe, vision‐threatening disorder characterized by the fibrous membrane formation that leads to trac‐tional retinal detachment. There has been no effective therapeutic approach other than vitreoretinal surgery. In this study, DNA microarray analysis of the fibrous membranes revealed significant up‐regulation of periostin. We also found increased periostin expression in the vitreous and retinal pigment epithelial (RPE) cells from fibrous membranes of PVR patients. In vitro , periostin increased proliferation, adhesion, migration, and collagen production in RPE cells through integrin αVmediated FAK and AKT phosphorylation. Periostin blockade suppressed migration and adhesion induced by TGFβ2 and PVR vitreous. In vivo , periostin inhibition had the inhibitory effect on progression of experimental PVR in rabbit eyes without affecting the viability of retinal cells. These results identified periostin as a pivotal molecule for fibrous membrane formation as well as a promising therapeutic target for PVR.—Ishikawa, K., Yoshida, S., Nakao, S., Nakama, T., Kita, T., Asato, R., Sassa, Y., Arita, R., Miyazaki, M., Enaida, H., Oshima, Y., Murakami, N., Niiro, H., Ono, J., Matsuda, A., Goto, Y., Akashi, K., Izuhara, K., Kudo, A., Kono, T., Hafezi‐Moghadam, A., Ishibashi, T. Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy. FASEB J. 28, 131–142 (2014). www.fasebj.org