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A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response
Author(s) -
Wilson Samantha L.,
Guilbert Marie,
SuléSuso Josep,
Torbet Jim,
Jeannesson Pierre,
Sockalingum Ganesh D.,
Yang Ying
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.13-227579
Subject(s) - fibrillogenesis , fibril , biophysics , chemistry , self healing hydrogels , biochemistry , polymer chemistry , biology
During aging, collagen structure changes, detrimentally affecting tissues' biophysical and biomechanical properties due to an accumulation of advanced glycation end‐products (AGEs). In this investigation, we conducted a parallel study of microscopic and macroscopic properties of differentaged collagens from newborn to 2‐yr‐old rats, to examine the effect of aging on fibrillogenesis, mechanical and contractile properties of reconstituted hydrogels from these collagens seeded with or without fibroblasts. In addition to fibrillogenesis of collagen under the conventional conditions, some fibrillogenesis was conducted alongside a 12‐T magnetic field, and gelation rate and AGE content were measured. A nondestructive indentation technique and optical coherence tomography were used to determine the elastic modulus and dimensional changes, respectively. It was revealed that in comparison to younger specimens, older collagens exhibited higher viscosity, faster gelation rates, and a higher AGE‐specific fluorescence. Exceptionally, only young collagens formed highly aligned fibrils under magnetic fields. The youngest collagen demonstrated a higher elastic modulus and contraction in comparison to the older collagen. We conclude that aging changes collagen monomer structure, which considerably affects the fibrillogenesis process, the architecture of the resulting collagen fibers and the global network, and the macroscopic properties of the formed constructs.—Wilson, S. L., Guilbert, M., Sulé‐Suso, J., Torbet, J., Jeannesson, P., Sockalingum, G. D., Yang, Y. A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response. FASEB J. 28, 14–25 (2014). www.fasebj.org