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Early methyl donor deficiency produces severe gastritis in mothers and offspring through N ‐homocysteinylation of cytoskeleton proteins, cellular stress, and inflammation
Author(s) -
BossenmeyerPourié Carine,
Pourié Grégory,
Koziel Violette,
Helle Deborah,
Jeannesson Elise,
Guéant JeanLouis,
Beck Bernard
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.12-224642
Subject(s) - inflammation , medicine , endocrinology , offspring , superoxide dismutase , gastric mucosa , apoptosis , biology , chemistry , immunology , oxidative stress , biochemistry , stomach , pregnancy , genetics
We examined the gastric mucosa structure and inflammatory status in control well‐nourished Wistar dams and in Wistar dams deprived of choline, folate, and vitamin B 12 during gestation and suckling periods, and in their offspring just before birth and at weaning. In this model of methyl donor deficiency (MDD), structural protein (E‐cadherin and actin) N ‐homocysteinylation was measured through immunoprecipitation and proximity ligation assays. Cellular stress, inflammation, and apoptosis were estimated by the analysis of the NF‐κB pathway, and the expression of superoxide dismutase, cyclooxygenase‐2, tumor necrosis factor α, caspases 3 and 9, and TUNEL assay. Aberrant gastric mucosa formation and signs of surface layer erosion were detected in MDD fetuses and weanlings. E‐cadherin and actin were N ‐homocysteinylated (+215 and +249% vs. controls, respectively; P <0.001). Expression of β‐catenin staining drastically decreased (–98%; P <0.01). NF‐κB pathway was activated (+124%; P <0.01). Expressions of all inflammatory factors (+70%; P <0.01), superoxide dismutase (+55%; P <0.01), and caspases (+104%; P <0.01) were markedly increased. These changes were also observed in dams, to a lesser extent. Early MDD induced gastric mucosa injury similar to atrophic gastritis through structural protein N ‐homocysteinylation, marked inflammation, and apoptosis, despite activation of repair machinery.—Bossenmeyer‐Pourié, C., Pourié, G., Koziel, V., Helle, D., Jeannesson, E., Guéant, J.‐L., and Beck, B. Early methyl donor deficiency produces severe gastritis in mothers and offspring through N ‐homocysteinylation of cytoskeleton proteins, cellular stress, and inflammation. FASEB J. 27, 2185–2197 (2013). www.fasebj.org