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Bacterial resistance mechanism: what proteomics can elucidate
Author(s) -
Lima Thais Bergamin,
Pinto Michelle Flaviane Soares,
Ribeiro Suzana Meira,
Lima Loiane Alves,
Viana Juliana Cançado,
Júnior Nelson Gomes,
Souza Cândido Elizabete,
Dias Simoni Campos,
Franco Octávio Luiz
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.12-221127
Subject(s) - antibiotics , antibiotic resistance , bacteria , proteomics , biology , mechanism (biology) , drug resistance , computational biology , microbiology and biotechnology , gene , genetics , philosophy , epistemology
Antibiotics are important therapeutic agents commonly used for the control of bacterial infectious diseases; however, resistance to antibiotics has become a global public health problem. Therefore, effective therapy in the treatment of resistant bacteria is necessary and, to achieve this, a detailed understanding of mechanisms that underlie drug resistance must be sought. To fill the multiple gaps that remain in understanding bacterial resistance, proteomic tools have been used to study bacterial physiology in response to antibiotic stress. In general, the global analysis of changes in the protein composition of bacterial cells in response to treatment with antibiotic agents has made it possible to construct a database of proteins involved in the process of resistance to drugs with similar mechanisms of action. In the past few years, progress in using proteomic tools has provided the most realistic picture of the infective process, since these tools detect the end products of gene biosynthetic pathways, which may eventually determine a biological phenotype. In most bacterial species, alterations occur in energy and nitrogen metabolism regulation; glucan biosynthesis is up‐regulated; amino acid, protein, and nucleotide synthesis is affected; and various proteins show a stress response after exposing these microorganisms to antibiotics. These issues have been useful in identifying targets for the development of novel antibiotics and also in understanding, at the molecular level, how bacteria resist antibiotics.—Lima, T. B., Pinto, M. F. S., Ribeiro, S. M., de Lima, L. A., Viana, J. C., Júnior, N. G., Cândido, E. D., Dias, S. C., and Franco, O. L. Bacterial resistance mechanism: what proteomics can elucidate. FASEB J. 27, 1291–1303 (2013). www.fasebj.org