Premium
Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity
Author(s) -
Degen Joachim,
Dublin Pavel,
Zhang Jiong,
Dobrowolski Radoslaw,
Jokwitz Melanie,
Karram Khalad,
Trotter Jacqueline,
Jabs Ronald,
Willecke Klaus,
Steinhäuser Christian,
Theis Martin
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.12-207183
Subject(s) - reporter gene , mutagenesis , transgene , astrocyte , gene , biology , genetically modified mouse , gene knockin , microbiology and biotechnology , computational biology , genetics , mutation , gene expression , neuroscience , central nervous system
Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre‐mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up‐regulation within gene families, and quantify Cre‐mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock‐in ECFP mouse revealed an unexpected heterogeneity of Cx43‐expressing astrocytes across brain areas.—Degen, J., Dublin, P., Zhang, J., Dobrowolski, R., Jokwitz, M., Karram, K., Trotter, J., Jabs, R., Willecke, K., Steinhäuser, C., Theis, M. Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity. FASEB J. 26, 4576–4583 (2012). www.fasebj.org