Premium
Group IVA phospholipase A 2 optimizes ovulation and fertilization in rodents through induction of and metabolic coupling with prostaglandin endoperoxide synthase 2
Author(s) -
Kurusu Shiro,
Sapirstein Adam,
Bonventre Joseph V.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.12-203968
Subject(s) - ovulation , medicine , human fertilization , endocrinology , human chorionic gonadotropin , prostaglandin , ovulation induction , biology , prostaglandin e2 , andrology , phospholipase a2 , chemistry , hormone , biochemistry , enzyme , anatomy
Female mice lacking group IVA phospholipase A2 ( Pla2g4a –/– ) have a smaller litter size, which is due, in part, to defective implantation. We examined PLA 2 G4A dependence of the processes of ovulation and fertilization. Following induction of ovulation by equine chorionic gonadotropin (eCG)/human CG (hCG) treatment and mating, ovulation and fertilization rates were reduced significantly in Pla2g4a –/– mice as compared to wild‐type littermates. Human CG triggered robust ovarian prostaglandin (PG) E 2 production in the preovulatory period that was significantly attenuated in Pla2g4a –/– mice. Human CG transiently enhanced ovarian expression of PLA2G4A and prostaglandin endoperoxide synthase 2 (PTGS2) in wild‐type mice. This PTGS2 induction was decreased in Pla2g4a –/– mice and also in immature rats treated with the PLA 2 G4A inhibitor, archidonyl trifluoromethyl ketone. A close spatiotemporal association of PLA 2 G4A with PTGS2 was found in mouse and rat preovulatory follicles examined by immunohistochemistry. Less association was observed with 4 other forms of PLA 2 . Our data strongly suggest that PLA2G4A amplifies hCG induction of PTGS2 and colocalizes with the induced PTGS2, thus contributing to robust PG production required for optimal ovulation and fertilization in rodents.—Kurusu, S., Sapirstein, A., Bonventre, J. V. Group IVA phospholipase A 2 optimizes ovulation and fertilization in rodents through induction of and metabolic coupling with prostaglandin endoperoxide synthase 2. FASEB J. 26, 3800–3810 (2012). www.fasebj.org