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Myc‐associated zinc finger protein (MAZ) is regulated by miR‐125b and mediates VEGF‐induced angiogenesis in glioblastoma
Author(s) -
Smits Michiel,
Wurdinger Thomas,
Hof Bert,
Drexhage Joost A. R.,
Geerts Dirk,
Wesseling Pieter,
Noske David P.,
Vandertop W. Peter,
Vries Helga E.,
Reijerkerk Arie
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-202820
Subject(s) - angiogenesis , cancer research , zinc finger , neovascularization , vascular endothelial growth factor , transcription factor , biology , chemistry , endocrinology , medicine , vegf receptors , gene , biochemistry
In patients with glioblastomas, vascular endothelial growth factor (VEGF) is a key mediator of tumor‐associated angiogenesis. Glioblastomas are notorious for their capacity to induce neovascularization, driving continued tumor growth. Here we report that miR‐125b is down‐regulated in glioblastoma‐associated endothelial cells, resulting in increased expression of its target, myc‐associated zinc finger protein (MAZ), a transcription factor that regulates VEGF. The down‐regulation of miR‐125b was also observed on exposure of endothelial cells to glioblastoma‐conditioned medium or VEGF, resulting in increased MAZ expression. Further analysis revealed that inhibition of MAZ accumulation by miR‐125b, or by MAZ‐specific shRNAs, attenuated primary human brain endothelial cell migration and tubule formation in vitro , phenomena considered to mimick angiogenic processes in vitro . Moreover, MAZ expression was elevated in brain blood vessels of glioblastoma patients. Altogether these results demonstrate a functional feed‐forward loop in glioblastoma‐related angiogenesis, in which VEGF inhibits the expression of miR‐125b, resulting in increased expression of MAZ, which in its turn causes transcriptional activation of VEGF. This loop is functionally impeded by the VEGF receptor inhibitor vandetanib, and our results may contribute to the further development of inhibitors of tumor‐angiogenesis.—Smits, M., Wurdinger, T., van het Hof, B., Drexhage, J. A. R., Geerts, D., Wesseling, P., Noske, D. P., Vandertop, W. P., de Vries, H. E., Reijerkerk, A. Myc‐associated zinc finger protein (MAZ) is regulated by miR‐125b and mediates VEGF‐induced angiogenesis in glioblastoma. FASEB J. 26, 2639‐2647 (2012). www.fasebj.org